MassIVE MSV000089881

Partial Public PXD035312

Vitamin C modulates the levels of several proteins of the mitochondrial complex III and its activity in the mouse liver

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Description

In the present study, we used the Gulo-/- female and male mice model which depends entirely on ascorbate derived from the diet. Importantly, the levels of ascorbate found in the serum of Gulo-/- mice reflect the amounts of ascorbate provided in drinking water. We tested different concentrations of ascorbate ranging from 0 to 0.4% (w/v) ascorbate, added into drinking water, until the age of four months. We performed label-free Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) global quantitative proteomic profiling to identify and quantify proteins in microsomal enriched liver extracts (MEE) from our different ascorbate treated cohorts of Gulo-/- females and males. We found that the MS intensities of several proteins in the mitochondrial complex III of the electron transport chain correlated positively with liver ascorbate concentrations in both Gulo-/- females and males. Consequently, the mitochondrial complex III activity in Gulo-/- female and male mice treated with suboptimal hepatic concentrations of ascorbate was significantly lower than Gulo-/- mice treated with optimal ascorbate concentration. Finally, proteins involved in complement activation correlated negatively with liver ascorbate concentrations in both Gulo-/- males and females. [doi:10.25345/C5Z892K19] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Vitamin C, gulonolactone oxidase, mass spectrometry, mitochondrial complex III, reactive oxygen species, sex-related differences, liver, mouse

Contact

Principal Investigators:
(in alphabetical order) 		Michel Lebel, Centre Hospitalier Universite Laval, Canada
Submitting User: MicLeb
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