MassIVE MSV000091101

Partial Public PXD039562

Systemic toxicity of snake venom metalloproteinases: proteomics analyses of kidney injury and blood plasma disturbances in a mouse model

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Description

Metalloproteinases are abundant in viperid venoms and are implicated in several envenomation effects. Bothrops jararaca venom is reported to induce kidney injury and coagulopathy. HF3 is an extremely hemorrhagic metalloproteinase from B. jararaca venom and participates in the local pathogenesis of envenomation. Here we applied proteomics approaches to evaluate the effects of HF3 in the mouse kidney and blood plasma after 2 h and 6 h of HF3 injection in the thigh muscle. All proteomic analyses were performed using orbitrap-based high-resolution mass spectrometry. For kidney tissue and plasma proteomic analysis, proteins were extracted and submitted to trypsin digestion and peptides were TMT-labeled for relative quantification. For N-terminomic analysis of kidney tissue, a modified Terminal Amine Isotopic Labeling of Substrates (TAILS) protocol was applied, using TMT-tags for free-amine blocking and relative quantification. [doi:10.25345/C55Q4RW6G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: snake venom effect ; mouse model ; metalloproteinase ; HF3 ; kidney ; plasma

Contact

Principal Investigators:
(in alphabetical order) 		Prof. Oliver Schilling, Institute for Surgical Pathology, University Medical Center Freiburg, Germany
Submitting User: miguelcos

Publications

Trevisan-Silva D, Cosenza-Contreras M, Oliveira UC, da Rós N, Andrade-Silva D, Menezes MC, Oliveira AK, Rosa JG, Sachetto ATA, Biniossek ML, Pinter N, Santoro ML, Nishiyama-Jr MY, Schilling O, Serrano SMT.
Systemic toxicity of snake venom metalloproteinases: Multi-omics analyses of kidney and blood plasma disturbances in a mouse model.
Int J Biol Macromol. 2023 Dec 31;253(Pt 6):127279. Epub 2023 Oct 6.

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