MassIVE MSV000079331

Partial Public PXD002973

Metabolic reprogramming in the airway epithelium of high risk individuals

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Description

We investigated proteins identified by shotgun proteomics in cytologically normal airway epithelial cells from individuals at different levels of risk for lung cancer. We identified 2869 proteins in bronchial brushings from individuals at low, moderate or high risk for lung cancer. Pathway analysis revealed enrichment of carbohydrate metabolic pathways in high risk individuals. Differential expression of selected proteins was validated by parallel reaction monitoring mass spectrometry in separate individual bronchial brushings. Augmentation of glucose consumption and lactate production measured in human bronchial epithelial cell BEAS2B treated with cigarette smoke condensate and increased synthetic ability and reductive carboxylation revealed by metabolic flux analysis indicated profound metabolic reprogramming. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: airway epithelium ; cigarette smoke ; metabolic reprogramming ; carbohydrate metabolism

Contact

Principal Investigators:
(in alphabetical order) 		Pierre Massion
Submitting User: rslebos

Publications

Rahman SM, Ji X, Zimmerman LJ, Li M, Harris BK, Hoeksema MD, Trenary IA, Zou Y, Qian J, Slebos RJ, Beane J, Spira A, Shyr Y, Eisenberg R, Liebler DC, Young JD, Massion PP.
The airway epithelium undergoes metabolic reprogramming in individuals at high risk for lung cancer.
JCI Insight. 2016 Nov 17;1(19):e88814. Epub 2016 Dec 17.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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