MassIVE MSV000084276

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GNPS_GC_NYC_fecal samples_SCFAs_MCFAs_derivatized

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Description

IL-17 Inhibition in Spondyloarthritis Associates with Subclinical Gut Microbiome Perturbations and a Distinctive IL-25-Driven Intestinal Inflammation. The data for medium chain fatty acids: Post-thawing, human fecal (50 mg) were sequentially extracted using solvent extraction with 100 uL 100% Methanol HPLC grade followed by sonication for 10 minutes and centrifugation (12000 rpm, 5 minutes). Supernatant was then transferred to a new 1.7 ml tube and the samples were lyophilized followed by resuspension in 50 uL MTBSTFA (N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-Butyldimethylchlorosilane). After sonication (5 minutes) and centrifugation (12000 rpm, 10 minutes) the prepared samples were placed in a 200 uL insert in 2 ml borosilicate vials and capped with a screw cap with silicone septum. The samples were stored at -20 C and thawed immediately prior to analysis. [doi:10.25345/C5T368] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: medium chain fatty acids ; GC-MS ; MTBSTFA ; derivatization

Contact

Principal Investigators:
(in alphabetical order) 		Pieter Dorrestein, UCSD, United States
Submitting User: aaksenov
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GNPS content goes here (MSV000084276 [task=9d06243175fe451c9a1d81c03fc14ae9])
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