IL-17 Inhibition in Spondyloarthritis Associates with Subclinical Gut Microbiome Perturbations and a Distinctive IL-25-Driven Intestinal Inflammation
Volatile short-chain fatty acids analysis.
Approximately 50 mg of sample were placed in 2 ml borosilicate vials capped with a screw cap with silicone septum. The capped vials were stored at -20 C and thawed immediately prior to analysis. The GC-MS analysis was carried out using the Agilent 7200 GC/QTOF equipped with robotic sampler system. The volatiles from the sample were extracted from headspace using Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) df 65 um Solid Phase Microextraction Fiber (SPME) for 30 minutes at 50 C. The fiber was then inserted into the injector equipped with Merlin septum heated to 250 C and the adsorbed compounds were desorbed for 1 minute.
[doi:10.25345/C5P94B]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: GC-MS ; SPME ; SCFA ; volatiles ; fecal
Principal Investigators: (in alphabetical order) |
Pieter Dorrestein, UCSD, United States |
Submitting User: | aaksenov |
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