All of the sampling has been conducted under UC San Diego IRB #171662. Cleaned and degassed PDMS patches have been placed on skin of a volunteer overnight and secured in place with waterproof bandage. After sampling, the bandage was removed, patches placed into 2 mL borosilicate vials using clean tweezers and 150 uL of HPLC grade methanol added for extraction. The extraction was carried out overnight at room temperature. After extraction, the sample was transferred into a 200 uL insert in a 2 mL vial and the vial capped with a crimp camp with silicone septum. The GC-MS analysis was carried out using the Agilent 7200 GC/QTOF equipped with robotic sampler system.
[doi:10.25345/C5195T]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: skin ; 3D mapping ; volatilome ; PDMS ; extraction ; methanol
Principal Investigators: (in alphabetical order) |
Pieter Dorrestein, University of California San Diego, USA |
Submitting User: | aaksenov |
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