MassIVE MSV000085238

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Decoding the protein composition of whole nucleosomes with Nuc-MS

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Description

Nuc-MS characterizes histone modifications and variants directly from intact endogenous mono-nucleosomes. Preserving whole nucleosome particles enables precise interrogation of their protein content, as for H3.3-containing nucleosomes which had 6-fold co-enrichment of variant H2A.Z over bulk chromatin. Moreover, Nuc-MS showed co-occurrence of oncogenic H3.3K27M with euchromatic marks (e.g., H4K16ac and >40-fold enrichment of H3K79me2). By capturing the entire epigenetic landscape, Nuc-MS provides a new, quantitative readout of nucleosome-level biology. [doi:10.25345/C5G11K] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: native mass spectrometry ; top-down ; epiproteomics

Contact

Principal Investigators:
(in alphabetical order) 		Neil L Kelleher, Northwestern University, United States
Submitting User: kelleheroffice
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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