Description
Combining RNA sequencing of breast cancer brain metastases with reverse genetic screen in Drosophila melanogaster, we identified Rab11b, an endosomal recycling protein, as a mediator of metastatic adaptation. We show that disseminated cells up-regulate Rab11b early after arrival in the brain, allowing control of the cell surface proteome through recycling of proteins required for successful interaction with the microenvironment, including integrin B1. Rab11b-mediated control of integrin B1 surface expression allows ligation to the brain ECM, activating mechanotransduction signaling to allow survival and proliferation. We propose a model in which up-regulation of Rab11b allows disseminated cells to recycle needed proteins during metastatic adaptation, without strictly requiring transcription and translation, to allow for metastatic outgrowth.
[doi:10.25345/C56M6V]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Brain cancer brain metastases ; Rab11b ; Proteomics ; Mass spectrometry
Contact
Principal Investigators:
(in alphabetical order)
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Dr. Siyuan Zhang, Indiana University Melvin and Bren Simon Cancer Center, USA
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uma_aryal
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Distinct condition labels are counted across all files submitted in the "Metadata" category
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Originally identified proteins that were automatically
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A protein is differentially abundant if its change in abundance across conditions is found
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
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