MassIVE MSV000093126

Partial Public PXD046246

TMT isobaric profiling of young versus old haematopoietic stem cells

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Description

Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spectra were used for peptide identification and quantitation based on TMT reporter ions. TMT isobaric comparison of old versus young haematopoietic stem and progenitor cells. Young 1 and Young 2 are samples 126 and 128 of dataset UTH_1. Old 1 and Old 2 are samples 129 and 130 of UTH_1. Young 3 is sample 131 and Old 3 is sample 130 of dataset UTH_4. [doi:10.25345/C59W0989X] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Cyclophilin A ; PPIA ; Aging

Contact

Principal Investigators:
(in alphabetical order) 		Andre Catic, Baylor College of Medicine, USA
Submitting User: acatic

Publications

Maneix L, Iakova P, Lee CG, Moree SE, Lu X, Datar GK, Hill CT, Spooner E, King JCK, Sykes DB, Saez B, Di Stefano B, Chen X, Krause DS, Sahin E, Tsai FTF, Goodell MA, Berk BC, Scadden DT, Catic A.
Cyclophilin A supports translation of intrinsically disordered proteins and affects haematopoietic stem cell ageing.
Nat Cell Biol. Epub 2024 Mar 29.

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