MassIVE MSV000093127

Partial Public PXD046247

TMT isobaric profiling of PPIA heterozygous versus knockout haematopoietic stem cells

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Description

Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spectra were used for peptide identification and quantitation based on TMT reporter ions. TMT isobaric comparison of Ppia heterozygous (het) versus knockout (KO) haematopoietic stem and progenitor cells. Het 1 is sample 126 and KO 1 is sample 131 of dataset UTH_2. Het 2 and KO 2 are samples 126 and 127 of UTH_4. Het 3 and KO 3 are samples 130 and 127 of dataset UTH_3. [doi:10.25345/C56688V8V] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Cyclophilin A ; PPIA ; Aging

Contact

Principal Investigators:
(in alphabetical order) 		Andre Catic, Baylor College of Medicine, USA
Submitting User: acatic

Publications

Maneix L, Iakova P, Lee CG, Moree SE, Lu X, Datar GK, Hill CT, Spooner E, King JCK, Sykes DB, Saez B, Di Stefano B, Chen X, Krause DS, Sahin E, Tsai FTF, Goodell MA, Berk BC, Scadden DT, Catic A.
Cyclophilin A supports translation of intrinsically disordered proteins and affects haematopoietic stem cell ageing.
Nat Cell Biol. Epub 2024 Mar 29.

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