MassIVE MSV000094752

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Metabolite profile at single-embryo level of 0 to 3h old Drosophila embryos

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Description

We used single-embryo metabolomics to characterize early developmental metabolism in Drosophila. We employed a multi-omics approach where samples were collected, homogenized in 80% methanol and the soluble fraction recover to perform targeted metabolomic whle RNA-seq was performed on the insoluble fraction to accurately stage each embryo. Then, this RNA-based staging was used to place single embryo metabolomes across the developmental trajectory. Thus, we are able to construct a highly detailed metabolomic map of embryonic development. Importantly, we validated our single-embryo metabolomics results in pools of 10 embryos. The data provide a continuous timeline of metabolite levels (and gene expression) during early development (0-3 hours) in Drosophila melanogaster. We used two genetically different lines from the Drosophila Genetic Reference Panel (DGRP) with known genetic variations in our crosses (males/DGRP_352, females/DGRP_737). RNA-seq data related to this dataset can be accessed at GEO under accession number GSE263568. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Development ; Embryo metabolism ; Maternal to zygotic transition ; Single-cell multi-'omics

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Principal Investigators:
(in alphabetical order) 		Adelheid Lempradl, Van Andel Institute, United States of America
Submitting User: jperezmojica
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