MassIVE MSV000094949

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Raw MS data - Proximity labeling of Sucrosomes

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Description

Human iPSCs (WTC11 line) expressing Lamp1-APEX were treated with sucrose overnight to form sucrosomes, and half of the sucrose-treated cells were treated with invertase to shrink sucrosomes to cause lysosome tubulation. Proximity labeling was done of the lysosomal surface as 3 technical replicates per condition. Biotinylated proteins were captured with NeutrAvidin Beads and samples were run by SDS-PAGE until they entered 1/4 the gel length. The short lanes were excised and processed for mass spectrometry by dissolving the gel and trypsin-digesting the samples. Label-free quantitation was performed. [doi:10.25345/C5SN01G1C] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: sucrosome ; invertase ; lysosome

Contact

Principal Investigators:
(in alphabetical order) 		Spencer Freeman, The Hospital for Sick Children, Canada
Submitting User: AmraSaric
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