MassIVE MSV000095322

Complete Public PXD053926

Understanding the genetic and structural bases of hornworts' carbon-concentrating mechanism

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Description

Proteomic measurements to identify putative Rubisco interacting proteins in hornworts compared to the green alga Chlamydomonas, the traditional model for studying pyrenoid-based CO2-concentrating mechanisms (pCCM). Using antibodies and immunoprecipitation against hornwort Rubisco large subunits to pull down Rubisco and any proteins that bind to it. Samples were digested with trypsin, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS processing pipeline against Anthoceros agrestis proteome. [doi:10.25345/C5M32NN3Q] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: hornworts ; CO2-concentrating mechanism ; proteomics ; LC-MS/MS

Contact

Principal Investigators:
(in alphabetical order) 		Fay-Wei Li, Boyce Thompson Institute for Plant Research, Cornell University, United States
Submitting User: alchemistmatt

Publications

Robison TA, Oh ZG, Lafferty D, Xu X, Villarreal JCA, Gunn LH, Li FW.
Hornworts reveal a spatial model for pyrenoid-based CO2-concentrating mechanisms in land plants.
Nat Plants. 2025 Jan;11(1):63-73. Epub 2025 Jan 3.

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