MassIVE MSV000096704

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The cryo-EM structure of mouse radial spoke 3 reveals a unique metabolic and regulatory hub in cilia

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Description

Samples are isolated axoneme from trachea cilia of mouse. Protein extracts were run into an SDS-PAGE gel and cut into 4 slices for each sample. LUM1_951105-951108: WT mouse, replicate 1 LUM1_951109-951112: AK7-deficient mouse, replicate 1 LUM1_1037650-1037653: WT mouse, replicate 2 LUM1_1037654-1037657: AK7-deficient mouse, replicate 2 LUM1_1069354-1069357: WT mouse, replicate 3 LUM1_1069358-1069361: AK7-deficient mouse, replicate 3 [doi:10.25345/C5N29PJ99] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Radial spoke ; respiratory cilia ; structure ; comparative proteomic analysis ; computational modeling ; atomic model ; phospho-regulation ; metabolic proteins ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Daniela Nicastro, UT Southwestern, United States
Submitting User: alemoff
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.