MassIVE MSV000097285

Partial Public PXD061697

Experiments using TurboID and BPA crosslinking to identify interactions between SNIPE, ManYZ, and phage proteins

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Description

TurboID was fused to different inner membrane proteins and proximity labeling + streptavidin bead pulldowns were used to identify biotinylated proteins. Separately, BPA was incorporated into SNIPE-FLAG and crosslinked to phage and host proteins with UV exposure, followed by anti-FLAG pulldowns and mass spectrometry to identify crosslinked proteins. [doi:10.25345/C5M61C26W] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: TurboID ; Phage defense ; Tape measure protein ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Michael Laub, Massachusetts Institute of Technology, United States of America
Submitting User: dsaxton
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Experimental Design
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Identification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.