MassIVE MSV000098428

Partial Public PXD065767

Characterization of NCLX knockout HeLa cells

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Description

Mass spectrometry was used as one of the complementary methods to validate the absence of NCLX in knockout HeLa cells. Purified, tryptic human NCLX peptides were analyzed by LC-MS/MS using DDA to generate a PRM inclusion list, which was then used to analyze mitochondrial peptides from knockout and wild-type HeLa cells to assess the presence of NCLX. LC-MS/MS was performed using an Orbitrap Fusion Tribid MS system (Thermo Fisher Scientific), and data was analyzed using Skyline (version 23.1.1.520; MacCoss Lab software) and FreeStyle™ 1.8 SP2 (Thermo Fisher Scientific). [doi:10.25345/C52B8VQ9Q] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: DatasetType:Proteomics ; PRM ; NCLX

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Principal Investigators:
(in alphabetical order) 		Liang Feng, Stanford University, USA
Submitting User: areiter
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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