MassIVE MSV000098614

Complete Public PXD066468

Characterization of a GpsB-associated regulator of PBP1a reveals the organization of the cell wall remodeling complex of Streptococcus pneumoniae

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Description

Class A PBPs (aPBPs) play a key role in the biosynthesis and remodeling of peptidoglycan, the main component of the bacterial cell wall. The human bacterial pathogen Streptococcus pneumoniae produces three aPBPs, which are regulated to maintain the ovoid shape of the bacterium. Although their exact functions remain unclear, evidence suggests that PBP1a and PBP2a activities are closely coordinated. In this study, we elucidated the function of an unknown function protein named GarP (GpsB-associated regulator of PBP1a), in the regulation of PBP1a activity. We showed that GarP localizes to the division septum and its absence leads to morphological defects. We further identified a GpsB-binding motif in GarP as well as in PBP2a, the PG deacetylase PgdA and the muramidase MpgA. Our analysis of genetic and protein interactions, combined with cell imaging, supports a model of a molecular complex that coordinates PG remodeling during S. pneumoniae cell division. [doi:10.25345/C5T14V26Z] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: peptidoglycan, cell wall, Streptococcus pneumoniae, cell division and morphogenesis, penicillin-binding proteins ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Christophe GRANGEASSE, MMSB-UMR5086-CNRS, France
Submitting User: fdelolme
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