MassIVE MSV000098905

Partial Public PXD067579

Effects of Mos-Kinase inhibition on protein levels during meiosis of starfish oocytes

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Description

The development of a haploid, fertilizable female gamete involves specialized meiotic divisions of the fully grown oocyte. An essential feature of oocyte meiosis is the reduction of genomic content to half, which is achieved by two successive divisions without an intervening S-phase. A known regulator of this event is a kinase called Mos that is exclusively expressed in oocytes during meiosis and activates the MEK/ERK/MAPK pathway. However, the downstream targets of the Mos-MAPK pathway and their significance in oocyte meiosis remains largely understudied. In this project, using phosphoproteomics on starfish (Patiria miniata) oocytes, we identify two predominant subsets of proteins that are targeted by Mos-MAPK at the critical time point - metaphase of meiosis I. These include proteins involved in CPE-mediated polyadenylation and cytoskeleton regulators. To complement this experiment, we performed DIA proteomics on Mos inhibited (20 micromolar U0126) and control oocytes at subsequent stages of meiosis (at 36 mins, 60 minutes and 90 minutes post hormone addition in U0126 inhibited oocytes and at metaphase I, meiosis I to meiosis II transition, metaphase II, 2 pronuclei and finally at the first mitotic division of the zygote for control oocytes). In this experiment, we identified 7,480 protein groups of which 5,103 protein groups could be quantified. We did not find significant changes in protein abundance across these time points and conditions, indicating that the proteome of starfish oocytes remains largely stable during meiosis. [doi:10.25345/C5TD9NN0W] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: meiosis, cytoskeleton, translational regulation, oocytes, Patiria miniata ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Henning Urlaub, Research Group Bioanalytical Mass Spectrometry, Max Planck Institute for Multidisciplinary Sciences; Bioanalytics, Department of Clinical Chemistry, University Medical Center Goettingen, Germany
Juliane Liepe, Research Group Quantitative and Systems Biology, Max Planck Institute for Multidisciplinary Sciences, Germany
Peter Lenart, Research Group Cytoskeletal Dynamics in Oocytes, Max Planck Institute for Multidisciplinary Sciences, Germany
Submitting User: Olexandr

Publications

Ivan Avilov, Yehor Horokhovskyi, Pooja Mehta, Luisa Welp, Jasmin Jakobi, Mingfang Cai, Aleksander Orzechowski, Henning Urlaub, Juliane Liepe and Peter Lenart.
Phosphoproteomic identification of Mos-MAPK targets in meiotic cell cycle and asymmetric division.
doi: 10.1083/jcb.202312140.

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