MassIVE MSV000100118

Partial Public PXD071637

DIA-PASEF proteomic profiling of cancer cells under MDM2 modulation, nutlin treatment, and poly(dA:dT) transfection

Subscribe Comment Convert Spectra Add Reanalysis

Description

Cell lysates from various treatment conditions were analyzed using DIA-PASEF quantitative proteomics. The study incorporated two distinct cell line models. SJSA1 cells: Treated with Nutlin or DMSO, following either Poly(dA:dT) transfection or a control transfection. H1299 cells: Engineered to overexpress either wild-type or mutant (6–200) MDM2 protein, followed by transfection with Poly(dA:dT) or a corresponding transfection control. [doi:10.25345/C58C9RH7D] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: MDM2, Nutlin, Poly(dA:dT), DIA-PASEF, SJSA-1 cells ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Prof. Henning Urlaub, Max Planck Institute for Multidisciplinary Sciences, Germany
Submitting User: sergey_moshkovskiy

Publications

Weber S, Manzini V, Dietrich H, Schotte H, Moshkovskii S, Hackert P, Duman ET, Vu NQ, Prokakis E, Salinas G, Lenz C, Urlaub H, Bohnsack KE, Bohnsack MT, Dobbelstein M.
MDM2 binds and suppresses RNA polymerase III to restrain the innate immune response to cytosolic DNA.
Cell Death Differ. Epub 2026 Aug 6.

Number of Files:
Total Size:
Spectra:
Subscribers:
 
Owner Reanalyses
Experimental Design
    Conditions:
    Biological Replicates:
    Technical Replicates:
 
Identification Results
    Proteins (Human, Remapped):
    Proteins (Reported):
    Peptides:
    Variant Peptides:
    PSMs:
 
Quantification Results
    Differential Proteins:
    Quantified Proteins:
 
Browse Dataset Files
Browse Metadata
 
FTP Download Link (click to copy):

Species

Instrument

Modifications

- Dataset Reanalyses

+ Dataset History

Click here to queue conversion of this dataset's submitted spectrum files to open formats (e.g. mzML). This process may take some time.

When complete, the converted files will be available in the "ccms_peak" subdirectory of the dataset's FTP space (accessible via the "FTP Download" link to the right).
Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.