MassIVE MSV000091053

Partial Public PXD039371

Identification of Trityl Post-translational modification by an untargeted bottom-up proteomic-based approach using UHPLC-Q Exactive Hybrid Quadrupole-Orbitrap high resolution mass spectrometry

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Description

We adopted an untargeted bottom-up proteomic-based approach with a high-resolution tandem-mass spectrometry for assessment of pesticide mixture induced neurotoxicity on human brain tumor meningiomas and in-depth elucidation of their involved mechanisms through protein-adduct formation or protein-Post translational modifications with prediction of alteration sites by de novo peptides sequencing. Three proteins namely, Glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), alpha-enolase (ENO1) and Phosphoglycerate-kinase-1 (PGK1) were selected as key targets in oxidative stress-response. Freshly resected human brain tumors were cultured in neural stem-cell culture medium for a 3D neurospheroid development model, then treated by pesticide mixture at relevant concentrations. Afterwards, proteins were extracted, reduced and alkylated, then digested by trypsin. The extracted peptides were analysed by a nano-UHPLC-ESI-Q Exactive HF hybrid quadrupole-Orbitrap high resolution mass spectrometry. Raw MS files were searched against human protein database based on the species of the samples using Maxquant (1.6.1.14). [doi:10.25345/C5CJ87W1H] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Nanow-flow UPLC ; tandem mass spectrometry (MS/MS) ; bottom-up proteomic-based approach ; Human brain tumors ; Neurospheroids ; PTMs ; triphenylmethyl ; pesticides

Contact

Principal Investigators:
(in alphabetical order) 		Kaouthar LOUATI, University of Monastir, Faculty of Pharmacy, Laboratory of Pharmacology, Analytics and Galenic Drug Development- LR12ES09, Road Avicenne 5000, Monastir, Tunisia, Tunisia
Submitting User: KAOUTHARLOUATI7

Publications

Kaouthar Louati,* Amina Maalej, Fatma Kolsi, Rim Kallel, Yassine Gdoura, Mahdi Borni, Leila Sellami Hakim, Rania Zribi, Sirine Choura, Sami Sayadi, Mohamed Chamkha, Basma Mnif, Zouheir Khemakhem, Tahya Sellami Boudawara, Mohamed Zaher Boudawara, Abderrahman Bouraoui, Jamil Kraiem, and Fathi Safta.
A shotgun proteomic-based approach with Q-Exactive Hybrid Quadrupole-Orbitrap High-Resolution mass spectrometer for assessment of pesticide mixture induced neurotoxicity on 3D-developed neurospheroid model from human brain meningiomas: Identification of trityl-post-translational modification.
https://doi.org/10.1021/acs.jproteome.4c00804.

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