MassIVE MSV000084830

Partial Public PXD017256

Autism-linked Cullin3 germline haploinsufficiency impacts cytoskeletal dynamics and cortical neurogenesis through RhoA signaling

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Description

E3-ubiquitin ligase Cullin3 (Cul3) is a high confidence risk gene for neurodevelopmental disorders including autism spectrum disorder (ASD) and developmental delay (DD). We generated Cul3-haploinsufficient mouse model to investigate brain anatomy, behavior, molecular, cellular, and circuit-level mechanisms dysregulated by Cul3 mutations. Spatiotemporal transcriptomic and proteomic profiling of the brain implicated neurogenesis and cytoskeletal defects as key drivers of Cul3 functional impact. Quantitative proteomic profiling was performed by Tandem Mass Tag (TMT) labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis with SPS-MS3 workflow. [doi:10.25345/C5509G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: TMT ; Cullin3 ; Autism ; Mouse brain

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Principal Investigators:
(in alphabetical order) 		Lilia Iakoucheva, UCSD, United States
Submitting User: namkyung
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