MassIVE MSV000085075

Complete Public PXD017980

Functional partitioning of a liquid-like organelle during assembly of axonemal dyneins

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Description

The associated files are the results of AP-MS experiments done from Xenopus multiciliated cells derived from dissected animal caps. Baits used for affinity purification were GFP-dnai2, GFP-wdr78, and GFP-C16orf71. Note that some files associated with the C16orf71 experiments may be mislabeled C16orf11 but reflect the C16orf71 protein nevertheless. Two sets of GFP pulldown controls were used for comparison, one for the dnai2 and wdr78 baits and a separate control for the C16orf71 experiment. The dnai2 and wdr78 sample sets were run on an Orbitrap Fusion and the C16orf71 samples were run on an Orbitrap Fusion Lumos mass spectrometer. [doi:10.25345/C5T69F] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: DynAPs ; AP-MS ; axonemal dynein ; cilia ; Xenopus ; DNAAFs

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Principal Investigators:
(in alphabetical order) 		Edward Marcotte, University of Texas-Austin, United States
Submitting User: OpheliaP
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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