MassIVE MSV000085456

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Presentation of the Extra-Cellular Domain of the Sars2 Spike Glycoprotein by Immunopeptidomic Profiling of HLA-II Bound Peptides from Human Dendritic Cells

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Description

In the present study we sought to identify the naturally processed and presented immunopeptidome of the SARS-CoV-2 spike glycoprotein from human antigen presenting cells. Monocyte-derived dendritic cells from a panel of healthy human subjects representing a majority of the HLA-DRB1 allele usage within the United States were treated with recombinant spike glycoprotein. A subset of donors was also selected to represent common alleles from the Asia- Pacific geography. HLA II associated peptides were identified by liquid chromatography and nanoelectrospray ionization tandem mass spectrometry after immunoprecipitation. [doi:10.25345/C51M7P] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: SARS-CoV-2 ; Immunopeptidomics ; dendritic cells ; HLA-II ; MAPPs ; spike glycoprotein ; human

Contact

Principal Investigators:
(in alphabetical order) 		Mike Knierman, Eli Lilly and Co., USA
Robert Siegel, Eli Lilly and Co., United States
Submitting User: mknierman

Publications

Knierman MD, Lannan MB, Spindler LJ, McMillian CL, Konrad RJ, Siegel RW.
The Human Leukocyte Antigen Class II Immunopeptidome of the SARS-CoV-2 Spike Glycoprotein.
Cell Rep. 2020 Dec 1;33(9):108454. Epub 2020 Nov 13.

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