In order to investigate cellular target proteins of EliF, a label-free target identification using thermal proteome profiling (TPP) was conducted.
Briefly, Hela cells were incubated with EliF-1b or EliF-2c, and each sample was divided into 10 fractions (high pH fractionation protocol).
A heat shock (ranges from 37C to 67C) was applied to each fraction to induce thermal denaturing. Non-denatured proteins were subsequently recovered, digested into peptides, and labeled with 10-plex TMT.
[doi:10.25345/C5697024Z]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: TPP ; fluorescent probe
Principal Investigators: (in alphabetical order) |
Jun-Seok Lee, Korea University College of Medicine, republic of korea |
Submitting User: | KyungTaeHong |
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