MassIVE MSV000095804

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COOKIE-Pro: Covalent Inhibitor Binding Kinetics Profiling on the Proteome Scale

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Description

COOKIE-Pro mass spec dataset collected from Orbitrap Lumos for spebrutinib SB2 (20231129_Speb_SB2_TMT18_ScaleUp_MS2_8ul.mzML; Ascend_VQneo_ES75500_SP2_X.mzML) and ibrutinib IB-C8-DTB (Lang_HFL_IB_COOKIE_X). Two-point COOKIE-Pro data are collected form Orbitrap Ascend, named as "Ascend_VQneo_ES75500_FAIMS_MS2_COOKIEX_Y". Two-point COOKIE-Pro data are searched with dynamic modification of unimod:1 (Carbamidomethyl) and unimod:2062 (DBIA) on cysteine. [doi:10.25345/C56D5PP33] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: covalent inhibitor ; BTK ; ibrutinib ; spebrutinib ; ABPP

Contact

Principal Investigators:
(in alphabetical order) 		Jin Wang, Baylor College of Medicine, Houston TX, USA
Submitting User: jinwang

Publications

Lin H, Yang B, Ding L, Yang YY, Holt MV, Jung SY, Zhang B, Wang MC, Wang J.
COOKIE-Pro: covalent inhibitor binding kinetics profiling on the proteome scale.
Nat Commun. 2025 Sep 30;16(1):8373. Epub 2025 Sep 30.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.