MassIVE MSV000099711

Complete Public PXD070166

MS-MS analysis of coomassie stained protein bands observed on the Alzheimer model active compound drug resin (102) versus control resin (74)

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Description

Pig brain was homogenized in column buffer supplemented with protease inhibitors and 10kx/g supernate prepared and flash frozen. Aliquot was thawed, 100ul applied to the 50ul bed volume drug resin (124) or control resin lacking the drug ligand (74) and incubated for 1hr at room temperature followed by 100 bed volumes of wash with column buffer. Free drug at 200uM in column buffer was used to elute bound proteins, and the drug resin stripped with 8M urea to remove proteins that may have stuck after elution. Samples 16-18 were the bands observed in the control resin. Samples 4-14 were the bands observed in the drug resin. Samples 1-3 were the bands observed in the 8M urea stripping of the drug resin and not observed upon similar stripping the control resin. [doi:10.25345/C56M33H2N] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Protein assembly modulation, allosteric site, small molecule, pig brain, Alzheimers Disease ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Vishwanath R. Lingappa, MD, PhD, Prosetta Biosciences, Inc, USA
Submitting User: M1chael_Ford
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