MassIVE MSV000092848

Partial Public PXD045348

Proteomic dataset of exosomes derived from fetal mesenchymal stromal cells

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Description

The raw files of the proteomics dataset are N=18 and a brief description of the files is shown below: AF1 raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 1 AF2.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 2 AFA1.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 3 AFA2.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 4 AFB1.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 5 AFB2.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 6 HL1A.raw: exosomes derived from Hepatocyte like cells 1 HL1B.raw: exosomes derived from Hepatocyte like cells 2 HL2A.raw: exosomes derived from Hepatocyte like cells 3 HL2B.raw: exosomes derived from Hepatocyte like cells 4 HL3A.raw: exosomes derived from Hepatocyte like cells 5 HL3B.raw: exosomes derived from Hepatocyte like cells 6 HPLA1.raw: exosomes derived from Hepatic Progenitor-like cells 1 HPLA2.raw: exosomes derived from Hepatic Progenitor-like cells 2 HPLB1.raw: exosomes derived from Hepatic Progenitor-like cells 3 HPLB2.raw: exosomes derived from Hepatic Progenitor-like cells 4 HPLA.raw: exosomes derived from Hepatic Progenitor-like cells 5 HPLB.raw: exosomes derived from Hepatic Progenitor-like cells 6 Raw files analysis was performed with Proteome Discoverer 1.4 (Thermo) software package, using the Sequest search engine and the Uniprot human (Homo sapiens) reviewed database, downloaded on December 15, 2017, including 20,243 entries. The search was performed using carbamidomethylation of cysteine as static and oxidation of methionine as dynamic modifications. Two missed cleavage sites, a precursor mass tolerance of 10 ppm and fragment mass tolerance of 0.05 Da were allowed. False discovery rate (FDR) validation was based on q value: target FDR : 0.05. Label free quantification was performed by utilizing the precursor ion area values exported from the total ion chromatogram as defined by the Proteome Discoverer v. 1.4.0.288 (Thermo Scientific). Output files from Proteome Discoverer were processed with R programming language for statistical computing (version 4.0.3). The following comparisons were made: AF vs HL, AF vs HPL and HL vs HPL. The msf files are N=18 and have the same name as the raw files above. [doi:10.25345/C5W669K46] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: mesenchymal stromal cells ; acute hepatic failure ; differentiation ; EVs ; secretome ; proteomics ; LC-MS/MS ; MFGE-8

Contact

Principal Investigators:
(in alphabetical order) 		Maria (Maya) Roubelakis, Laboratory of Biology, Medical School of Athens National and Kapodistrian University of Athens and Center of Basic Research Biomedical Research Foundation of the Academy of Athens, Greece
Submitting User: mmakrid

Publications

Adriana Psaraki , Dimitra Zagoura , Lydia Ntari , Manousos Makridakis , Christina Nikokiraki , Ourania Trohatou , Konstantina Georgila , Christos Karakostas , Ioanna Angelioudaki , Anastasios G Kriebardis , Roberto Gramignioli , Stratigoula Sakellariou , Maria Xilouri , Aristides G Eliopoulos , Antonia Vlahou , Maria G Roubelakis.
MFGE-8 identified in fetal mesenchymal-stromal-cell-derived exosomes ameliorates acute hepatic failure pathology.
iScience . 2023 Sep 30;26(11):108100. doi: 10.1016/j.isci.2023.108100. eCollection 2023 Nov 17.

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