MassIVE MSV000096255

Complete Public PXD057369

Development of subunit-selective proteasome substrates for Schistosoma species by MSP-MS

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Description

Development of optimized subunit-specific substrates for S. mansoni proteasome (Sm20S) based on data generated by Multiplex Substrate Profiling by Mass Spectrometry (MSP-MS). MSP-MS was performed with a library of 228 synthetic tetradecapeptides. Sm20S was incubated for 1h and 3h with Bortezomib, Carfilzomib, DMSO (vehicle control), and the absence of inhibitor or vehicle control (time 0 samples). Assays were conducted in quadruplicate by incubating Sm20S with the peptide library. [doi:10.25345/C5MC8RT6P] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: S. mansoni ; Proteasome ; Substrate ; Bortezomib ; Carfilzomib ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Anthony ODonoghue, University of California San Diego, USA
Submitting User: elanybs
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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