MassIVE MSV000085085

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GNPS - 20200311_Pseudomonas_psychrophila_sp_JB418_MS2

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Description

P. psychrophila sp. JB418 was grown on 10% Cheese Curd Agar (CCA) for 3 days prior to extraction. For extraction, Acetonitrile was added to each culture and sonicated for 1hr, then solvent was removed and dried to afford a dry weighed extract. Extracts were then dissolved in either 100% (pooled replicates) or 90% (individual replicates) Methanol and LC-MS/MS was performed. Pooled replicate was run on a Bruker Impact II qTOF and individual biological replicates were run on a Bruker compact qTOF. Solvent B was ACN with 0.1% Formic Acid and Solvent A was water with 0.1% Formic Acid for both analyses, gradient of 10-100% B over the course of 10-12 minutes on a UPLC C18 column. File naming: JB418_alone = pooled biological replicate, JB418 followed by number = individual biological replicates, CCA = media controls and MeOH = solvent blanks. [doi:10.25345/C5HQ3J] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Pseudomonas psychrophila

Contact

Principal Investigators:
(in alphabetical order) 		Laura Sanchez, University of Illinois at Chicago, USA
Submitting User: jess_cleary
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Identification Results
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GNPS content goes here (MSV000085085 [task=050edfa14f344319a33ee576a93b41a0])
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.