MassIVE MSV000096980

Complete Public PXD060281

Immunoglobulin A Carries Sulfated and O-acetylated N-glycans Primarily at the Tailpiece Site

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Description

A site-specific description of a recently discovered HexNAc-sulfated N-glycans of human immunoglobulin A (IgA) was still pending. Here we provide an in-depth N-glycoproteomic analysis of human serum IgA. This in-depth N-glycoproteomic analysis, developed by us, integrates a new strategy for identifying sulfated and other rare N-glycans in IgA. In our study, we implemented two strategies for identifying the new N-glycan compositions: screening for rare glycopeptides using oxonium marker ions and wildcard search to identify glycans holding a rare modification attached to peptides. The dataset provided here contains primarily IgA N-glycopeptides identified from technical quadruplicates of two commercial human serum IgA samples. A comparison of the N-glycosylation profiles of two commercial human serum IgA samples demonstrated that sulfated N-glycans are mainly present in the tailpiece site. Also, complex-type N-glycan compositions with O-acetylated sialic acid were identified in the tailpiece. Surprisingly, N-glycans bearing glucuronic acid were identified in the commercial IgA samples, but from peptides of contaminant glycoproteins.These N-glycans have not been included in previously published IgA micro-heterogeneity analyses. We expect that a broader micro-heterogeneity description of clinically relevant glycoproteins, such as IgA, can expand the screening for biomarkers or treatment options. [doi:10.25345/C5MP4W084] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: immunoglobulin A (IgA), mass spectrometry, glycoproteomics, oxonium marker ions, sulfation, O-acetylation, rare N-glycans ; N-glycosylation ; DatasetType:Other (glycoproteomics)

Contact

Principal Investigators:
(in alphabetical order) 		Erdmann Rapp, Max Planck Institute for Dynamics of Complex Technical Systems, Germany
Frania Jaqueline Zuniga-Banuelos, Max Planck Institute for Dynamics of Complex Technical Systems, Germany
Greta Lemke, Max Planck Institute for Dynamics of Complex Technical Systems, Germany
Marcus Hoffmann, Max Planck Institute for Dynamics of Complex Technical Systems, Germany
Udo Reichl, Max Planck Institute for Dynamics of Complex Technical Systems, Germany
Submitting User: Zuniga
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