MassIVE MSV000095745

Partial Public PXD055484

QCeltis Case Study 3: Dried Blood Spots QC Dataset

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Description

A dataset containing dried blood spots from human blood used as quality control samples to monitor variation, used as technical replicates (TR), digestion replicates (DR) and HeLa samples. Dried blood spots are blood from a finger prick spotted onto Whatman cards and dried. An aliquot of pooled, mixed-gender blood sample (Golden West Biosolutions, LLC., Human Whole Blood Lot# PS1000.104) were spotted on the same Whatman cards and used as DRs. 6 DRs were added to each digestion plate to be digested with the cohort data. During LC-MS acquisition, the 6 DRs are run at the beginning of each batch. In addition, the pooled, mixed-gender blood samples were bulk digested and included as TRs. 2 TRs were run for every acquisition batch, with the goal of assessing LC-MS performance. To further this goal, 2 HeLa samples were also run between every 2 MS batches. [doi:10.25345/C5PR7N58X] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Quality Control ; DIA ; Technical Replicates ; Digestion Replicates ; HeLa ; Dried Blood Spots

Contact

Principal Investigators:
(in alphabetical order) 		Jennifer Van Eyk, Cedars Sinai Medical Center, United States
Submitting User: vegesnam

Publications

Vegesna M, Sundararaman N, Bharadwaj A, Washington K, Pandey R, Haghani A, Chazarin B, Binek A, Fu Q, Cheng S, Herrington D, Van Eyk JE.
Enhancing Proteomics Quality Control: Insights from the Visualization Tool QCeltis.
J Proteome Res. 2025 Mar 7;24(3):1148-1160. Epub 2025 Feb 24.

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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.