The high throughput characterization of protein N-termini is becoming an emerging challenge in the proteomics and proteogenomics fields. The present study describes the N-terminal oriented analysis of human mitochondria enriched samples by the trimethoxyphenyl phosphonium (TMPP) labeling approach (N-TOP and dN-TOP approaches (Gallien et al, 2009, Bertaccini et al. 2013)). The dN-TOP strategy based on a double light/heavy TMPP labeling has been optimized in order to improve and automate the workflow for efficient, fast and reliable high throughput N-terminome analysis.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Free N-terminome analysis ; Human Mitochondria ; dN-TOP approach ; Proteogenomics
Principal Investigators: (in alphabetical order) |
Dr Alain Van Dorsselaer |
Submitting User: | ccms |
Vaca Jacome AS, Rabilloud T, Schaeffer-Reiss C, Rompais M, Ayoub D, Lane L, Bairoch A, Van Dorsselaer A, Carapito C.
N-terminome analysis of the human mitochondrial proteome.
Proteomics. 2015 Jul;15(14):2519-24. Epub 2015 Jun 8.
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