MassIVE MSV000084104

Imported Reanalysis Dataset Public PXD012985

Translational regulation contributes to the secretory response of chondrocytic cells following exposure to Interleukin-1B

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Description

Chondrocytes undergo changes to their protein translational capacity during osteoarthritis progression, but a study of how disease-relevant signals affect chondrocyte protein translation at the transcriptome level has not previously been performed. In this study we describe how the inflammatory cytokine IL-1B rapidly affects protein translation in the chondrosarcoma cell line SW1353. Using ribosome profiling we demonstrate that IL-1B induced altered translation of inflammatory-associated transcripts through differential translation and the use of multiple open reading frames. Proteomic analysis of the cellular layer and the conditioned media of these cells identified that proteins which were differentially translated were most readily detected in the secretome. We have produced combined ribosome profiling and proteomic datasets which provide a valuable resource in understanding the processes that are occuring during cytokine stimulation of chondrocytic cells. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Human ; chondrocytes ; ribosome profiling ; interleukin-1B ; inflammation ; secretion

Contact

Principal Investigators:
(in alphabetical order) 		Dr Simon Tew, Comparative Musculoskeletal Sciences Research Group Department of Musculoskeletal Biology Institute of Ageing and Chronic Disease University of Liverpool, N/A
Submitting User: ccms

Publications

McDermott BT, Peffers MJ, McDonagh B, Tew SR.
Translational regulation contributes to the secretory response of chondrocytic cells following exposure to interleukin-1?.
J Biol Chem. 2019 Aug 30;294(35):13027-13039. Epub 2019 Jul 12.

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