MassIVE MSV000086010

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Electroblotting through a tryptic membrane for LC-MS/MS analysis of proteins separated in electrophoretic gels

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Description

Electroblotting proteins separated by SDS-PAGE through a trypsin-containing membrane and onto a capture membrane simplifies extraction and digestion of proteins. Subsequent liquid chromatography-tandem mass spectrometry (LC-MS/MS) identifies the extracted peptides. [doi:10.25345/C54J3D] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: proteomics ; electroblotting ; protein seperation ; Trypsin ; SDS-PAGE

Contact

Principal Investigators:
(in alphabetical order) 		Adrianna Bickner, University of Notre Dame, United States
Submitting User: abickner

Publications

Bickner AN, Champion MM, Hummon AB, Bruening ML.
Electroblotting through a tryptic membrane for LC-MS/MS analysis of proteins separated in electrophoretic gels.
doi: 10.1039/d0an01380c.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.