A mass spectrometry-based proteomics analysis was performed to study the protein binders of GTSF1L in germ cell culture (BmN4; ovary-derived) of Bombyx mori. Anti-HA pull-downs were performed in BmN4 cells transfected with the HA-BmGtsf1L plasmid or the HA-eGFP control plasmid. Proteins were digested in-gel by trypsin. The resultant peptides were then dimethyl labelled and combined. Peptides were measured on a Q Exactive Plus Orbitrap mass spectrometer.
[doi:10.25345/C5474721Z]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Protein-protein interactions ; germline ; Bombyx mori ; dimethyl-labelling
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Principal Investigators: (in alphabetical order) |
Rene Ketting, Institute of Molecular Biology, Mainz, Germany |
| Submitting User: | imbpcf |
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