Comparison of off target alkylation using chloroacetamide (CAA) and iodoacetamide (IAA). The peptide preparation was performed side by side, following identical methods except the alkylation treatment with either IAA or CAA. None of the comparison of off-targets on between IAA and CAA showed a significant difference. The overall off-target level for both reagents was relatively low (< 0.1%) and the off-target rate on lysine residues was even lower (0.045% for IAA and 0.051% for CAA).
[doi:10.25345/C57W67H6F]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: iodoacetamide ; chloroacetamide
Principal Investigators: (in alphabetical order) |
Justin Walley, Iowa State University, US |
Submitting User: | jwalley |
Song G, Montes C, Olatunji D, Malik S, Ji C, Clark NM, Pu Y, Kelley DR, Walley JW.
Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis.
Plant Cell. Epub 2024 Nov 21.
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