MassIVE MSV000096773

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Freshwater Assembly Experiment (Exudates 2024) - Jackrel Lab

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Description

With the intent to understand the drivers of microbial assembly, we used phytoplankton as a model host organism. The external metabolome of thirty-one strains of freshwater algae were collected to better understand the drivers of interactions between phytoplankton hosts and bacteria. To collect the metabolite profile, sterile monocultures of the 31 phytoplankton strains were grown under identical conditions. When each strain reached carrying capacity, the culture was filtered through a 0.22um filter, removing all phytoplankton cells. This filtrate was then acidified to a pH of 2.5 and stored at 4C for 2-29 hours, until it could be processed through the solid phase extraction column. To prep the column, 1 cartridge volume (1mL) of 100% methanol was passed through the column. Once the column was prepped, 50mL of cell-free, acidified culture was passed through the column to collect the organic material. The column was cleaned with 2 cartridge volume of 0.01M HCl and air dried for 5 minutes. The sample was eluted into a deep-well plate over dry ice with 1mL of 100% methanol. The plates were stored at -80C until ready for processing. [doi:10.25345/C5QJ7894G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: MScollaboratory ; Phytoplankton ; Exudate ; Axenic ; DatasetType:Metabolomics

Contact

Principal Investigators:
(in alphabetical order) 		Sara Jackrel, University of California, San Diego, United States
Submitting User: mcmk3
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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