How cuticular proteins (CPs) interact with chitin and with each other in the cuticle remains unresolved. We employed LC-MS/MS to identify CPs from 5-6 day-old adults of Anopheles gambiae released after serial extraction with PBS, EDTA, 2-8M urea, and SDS as well as those that remained unextracted. Results were compared to published data on time of transcript abundance, localization of proteins within structures and within the cuticle, as well as properties of individual proteins, length, pI, percent histidine, tyrosine, glutamine, and number of AAP[A/V/L] repeats. Thirteen proteins were solubilized completely, all were CPRs. Eleven were identified in both soluble fractions and the final pellet, including 5 from other CP families. Forty-four were only detected from the final pellet. These included CPRs and members of the CPAP1, CPF, CPFL, CPLCA, CPLCG, CPLCP, and TWDL families, as well as several low complexity CPs, not assigned to families. For a given protein, many histidines or tyrosines or glutamines appear to be potential participants in cross-linking since we could not identify consistent absence of individual peptides bearing these residues. We failed to recover peptides from the amino-terminus of any CP. Whether this implicates that location in sclerotization or some modification that prevents detection is not known. Soluble CPRs had lower isoelectric points than those that remained in the final pellet; most members of other CP families had isoelectric points of 8 or higher.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: cuticular proteins ; anopheles gambiae ; Serial Extraction ; mosquito ; LC-MS/MS ; chitin
Principal Investigators: (in alphabetical order) |
Dr. Judith H. Willis, University of Georgia, United States of America |
Submitting User: | majorsbad |
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