MassIVE MSV000100313

Complete Public PXD072329

Proteomic analysis of redox PTMs in HCoV-229E-infected immortalized human lung epithelial cells (A549)

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Description

TMT18-labeled proteome-wide profiling of post-translational modifications (redox) in mock-infected and infected human lung epithelial cells (A549). Human lung epithelial (A549) cells were infected and mock-infected with HCoV-229E. Cells were harvested at 8, 16, and 24 hours post-infection. Extracted proteins were digested with LysC + Trypsin, then TMT18-labeled and enriched for redox PTMs. Samples were split into 6 fractions and run on the Orbitrap Exploris 480. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline. [doi:10.25345/C52R3P962] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: HCoV-229E ; coronavirus ; human lung ; post-translational modifications ; redox ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Amy Sims, Pacific Northwest National Laboratory, United States
Tong Zhang, Pacific Northwest National Laboratory, United States
Submitting User: alchemistmatt
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