MassIVE MSV000083362

Partial Public PXD012496

The N-cadherin interactome in primary cardiomyocytes as defined by quantitative proximity proteomics

Description

Low levels of Cdh2-BioID2 were expressed in mouse primary neonatal cardiomyocytes using an adenoviral expression system. Infected cells were treated with biotin for 24 hours before lysis. Biotinylated proteins were precipitated from lysates using streptavidin beads. Protein samples were in-gel trypsin digested and the resulting tryptic peptides were analyzed on an LTQ Orbitrap Velos. [doi:10.25345/C5VC9G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Adherens junction ; Cardiomyocyte ; Cdh2 ; Interactome ; Intercalated disc ; N-cadherin ; Proteomics

Contact

Principal Investigators: Adam V. Kwiatkowski, University of Pittsburgh, United States
Submitting User: zengxx

Publications

Li Y, Merkel CD, Zeng X, Heier JA, Cantrell PS, Sun M, Stolz DB, Watkins SC, Yates NA, Kwiatkowski AV.
The N-cadherin interactome in primary cardiomyocytes as defined by quantitative proximity proteomics.
J. Cell. Sci. Epub 2019 Jan 10.

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