Low levels of Cdh2-BioID2 were expressed in mouse primary neonatal cardiomyocytes using an adenoviral expression system. Infected cells were treated with biotin for 24 hours before lysis. Biotinylated proteins were precipitated from lysates using streptavidin beads. Protein samples were in-gel trypsin digested and the resulting tryptic peptides were analyzed on an LTQ Orbitrap Velos.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Adherens junction ; Cardiomyocyte ; Cdh2 ; Interactome ; Intercalated disc ; N-cadherin ; Proteomics
|Principal Investigators:||Adam V. Kwiatkowski, University of Pittsburgh, United States|
Li Y, Merkel CD, Zeng X, Heier JA, Cantrell PS, Sun M, Stolz DB, Watkins SC, Yates NA, Kwiatkowski AV.
The N-cadherin interactome in primary cardiomyocytes as defined by quantitative proximity proteomics.
J. Cell. Sci. Epub 2019 Jan 10.
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