MassIVE MSV000083362

Partial Public PXD012496

The N-cadherin interactome in primary cardiomyocytes as defined by quantitative proximity proteomics

Description

Low levels of Cdh2-BioID2 were expressed in mouse primary neonatal cardiomyocytes using an adenoviral expression system. Infected cells were treated with biotin for 24 hours before lysis. Biotinylated proteins were precipitated from lysates using streptavidin beads. Protein samples were in-gel trypsin digested and the resulting tryptic peptides were analyzed on an LTQ Orbitrap Velos. [doi:10.25345/C5VC9G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Adherens junction ; Cardiomyocyte ; Cdh2 ; Interactome ; Intercalated disc ; N-cadherin ; Proteomics

Contact

Principal Investigators:
(in alphabetical order)
Adam V. Kwiatkowski, University of Pittsburgh, United States
Submitting User: zengxx

Publications

Li Y, Merkel CD, Zeng X, Heier JA, Cantrell PS, Sun M, Stolz DB, Watkins SC, Yates NA, Kwiatkowski AV.
The N-cadherin interactome in primary cardiomyocytes as defined by quantitative proximity proteomics.
J. Cell. Sci. Epub 2019 Jan 10.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.