MassIVE MSV000096795

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The embryonic DPPA3 gene stimulates the expression of pregnancy-related genes in bovine endometrial cells

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Description

Extracellular vesicles (EVs) released by cells contain mRNAs, miRNAs, lncRNAs, lipids, and proteins, playing crucial roles in cell-cell communication. While full-length mRNA transcripts have been documented in EVs secreted by cancer cells, there are no reports on full transcripts secreted by embryos. Our study aimed to identify EV mRNAs in the culture media of bovine embryos and investigate their roles in embryo-mother communication. Following the isolation of EVs from in-vitro fertilization media samples and RNA sequencing, we identified a full mRNA transcript of DPPA3, known to play an essential role in embryo development. To examine the role of DPPA3 in embryo-mother communication, an in vitro transcribed mRNA of DPPA3 was transfected into bovine endometrial epithelial cells. Transfected and control cells were subsequently analyzed with RNA sequencing and proteomics to assess the effects of DPPA3 on gene expression. A total of 24 genes were found to be upregulated, and one gene was downregulated (FDR < 0.01) following DPPA3 transfection, many with known functions in pregnancy recognition. Proteomic analysis revealed 28 differentially expressed, with 19 upregulated and 15 downregulated. Two proteins, ISG15 and MX1, overlapped with the differentially expressed mRNAs. To mimic the natural transfer of EVs from embryos to endometrial cells, we performed co-culture with day-8 blastocysts or supplemented the cells with embryo-conditioned culture media. DPPA3 presence was detected in endometrial cells exposed to embryo-conditioned media after just 30 minutes. Overall, our study highlights the significant role of EVs in cell-cell communication through mRNA signaling from the embryo to the mother. [doi:10.25345/C5W37M739] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: DPPA3, embryo-mother communication, extracellular vesicles, endometrium, mRNA ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Nagib Ahsan, University of Oklahoma, United States
Submitting User: naproteomics
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