MassIVE MSV000087516

Complete Public PXD026262

Cold-Induced Adaptations to the Proteome of Mouse Subcutaneous White Adipose Tissue (scWAT) Reveal Proteins Relevant for Tissue Remodeling and Plasticity

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Description

White adipose tissue (WAT) is one of the most dynamic and plastic organs or tissues in the body, capable of expanding its mass in response to positive energy balance (either by proliferation of adipocytes - hyperphasia, or by increasing adipoycyte size - hypertrophy), developing inducible brown adipocytes through a process termed "browning", and changing its mitochondrial mass in response to stimuli such as cold exposure. Most notably, cold stimulation and release of norepinephrine from sympathetic nerve terminals is able to drive lipolysis in WAT (and thus smaller lipid droplet size and adipocyte size), as well as promote sympathetic nerve branching and increased neurite density, as well as development of pockets of browning around these neurite-dense regions of tissue. While several RNA-sequencing (including newer single cell RNAseq studies) have been done in WAT across situations of positive energy balance (such as high fat diet, obesity) or negative energy balance (such as cold stimulation of energy expenditure), very little work has been done to investigate changes to the adipose proteome while the tissue is actively remodeling. Here, we have carried out proteomics analysis on inguinal subcutaneous (sc)WAT of C57BL/6 mice after 24 or 72hrs of cold stimulation, a period during which UCP1 is actively turned on during the browning process. Through these data, we have found that numerous protein pathways changed by cold stimulation relate to tissue remodeling and plasticity, including changes to mitochondrial dynamics, tissue immune cells, and peripheral nerves. [doi:10.25345/C5PV57] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: white adipose tissue (WAT) ; browning ; cold ; mitochondria ; thermogenesis ; metabolism ; remodeling ; peripheral nerves ; glia ; cytoskeleton ; extracellular matrix ; bottom-up proteomics ; Q-Exactive HF-X

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Principal Investigators:
(in alphabetical order) 		Kristy L. Townsend, The Ohio State University, United States
Submitting User: gardner207
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