MassIVE MSV000093718

Complete Public PXD048064

Global phosphoproteomics for CRISPR/Cas9 mediated PTPN22 Ser325 mutagenesis on Jurkat cells

Description

LQF of 3x FLAG PTPN22 WT Jurkat cells compared to S325A and S325E KI mutant Jurkat cells. WT or KI lines were stimulated with or without TCR engagement for 5 min. [doi:10.25345/C5Q23RB0F] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: PTPN22 ; Protein tyrosine kinase ; Protein tyrosine phosphatase ; T cell signaling ; Phosphoproteomics ; Jurkat cells

Contact

Principal Investigators:
(in alphabetical order)
David J Gonzalez, UCSD, US
Nunzio Bottini, ACTRI, United States
Submitting User: leighanarossitto

Publications

Zhuang C, Yang S, Gonzalez CG, Ainsworth RI, Li S, Kobayashi MT, Wierzbicki I, Rossitto LM, Wen Y, Peti W, Stanford SM, Gonzalez DJ, Murali R, Santelli E, Bottini N.
A novel gain-of-function phosphorylation site modulates PTPN22 inhibition of TCR signaling.
J Biol Chem. 2024 Jun;300(6):107393. Epub 2024 May 21.

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