MassIVE MSV000099913

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S. elongatus PCC 7942 Limited Proteolysis (LiP) Proteomics - Light vs Dark with Reduced/Non-reduced preparations in Dense Cultures

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Description

LiP experimental assays investigating structural alterations in Dense Light vs Dense Dark growth conditions with Reduced/Non-reduced preparations. Samples are Synechococcus elongatus PCC7942 cell lysates (BTO:0004304). Purpose: Probing light- and dark-induced conformational changes in dense cyanobacterial cultures using reduced and non-reduced sample preparation protocols. Technique: Limited proteolysis (LiP). Other details: Nonspecific protease used was pronase at an enzyme:substrate ratio of 1:200. The reduced samples were treated with 100 mM DTT prior to LiP. Data was searched with MaxQuant (using Label free quantification- Match between runs, LFQ-MBR). [doi:10.25345/C53R0Q688] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Limited Proteolysis ; LiP ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		John T. Melchior, Pacific Northwest National Laboratory, USA
Snigdha Sarkar, Pacific Northwest National Laboratory, USA
Submitting User: alchemistmatt
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