MassIVE MSV000099293

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Top-Down Mass Spectrometry Reveals Alterations in Histone Proteoforms by Human Coronavirus 229E infection

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Description

Top-down proteomic data from enriched histones samples from MRC-5 cell. Samples were infected with human coronavirus 229E for 24 hours and compared against mock samples using differential abundance analysis (N=5 per condition). Intact histone samples were analyzed by reversed phase-liquid chromatography MS/MS. Data was searched with TopPIC using PNNL's DMS processing pipeline and post-processed with TopPICR. [doi:10.25345/C59G5GS7H] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: histone ; truncation ; top-down proteomics ; human coronavirus 229E ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Amy C. Sims, Pacific Northwest National Laboratory, United States
Tong Zhang, Pacific Northwest National Laboratory, United States
Submitting User: alchemistmatt
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.