MassIVE MSV000080265

Imported Reanalysis Dataset Public PXD003664

Serial interactome capture of the human cell nucleus

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Description

Novel RNA-guided cellular functions are paralleled by an increasing number of RNA binding proteins (RBPs). We present “serial interactome capture” (serIC), a multiple purification procedure of UV-crosslinked poly(A)-RNA-protein complexes that enables global RBP detection with maximal specificity. We apply serIC to nuclei of proliferating K562 cells to obtain the first human nuclear interactome. The domain composition of the 382 identified nuclear RBPs markedly differs from previous IC experiments, including fewer factors without known RNA binding domains that are in better agreement with computationally predicted RNA binding. serIC extends the number of DNA-RNA binding proteins (DRBPs), and reveals a network of RBPs involved in p53 signaling and double strand break repair. serIC is an effective tool to couple global RBP capture with additional selection or labelling steps for specific detection of highly purified RBPs. The nuclear interactome presented here is a stepping-stone towards deciphering of the functional RNA-protein network in the mammalian nucleus. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: RNA binding proteins ; interactome capture ; nuclear RNA binding proteome

Contact

Principal Investigators:
(in alphabetical order) 		Dr Ulf �rom
Submitting User: ccms

Publications

Conrad T, Albrecht AS, de Melo Costa VR, Sauer S, Meierhofer D, Ørom UA.
Serial interactome capture of the human cell nucleus.
Nat Commun. Epub 2016 Apr 4.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.