Thirty commercial proteins were prepared at 1.5 pmol/µL in three different subsets of 10 proteins each. Proteins from these subsets were spiked to a 15-µg Escherichia coli background in different amounts and five different mixtures were prepared in triplicates. The final amount of each protein in each mixture was either 100, 200 or 400 fmol/µg of E. coli background for the subsets marked as 1, 2 and 4, respectively. The generated dataset with known concentrations of spiked-in proteins was used to evaluate the performance of several processing tools of MS proteomics datasets.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: MSstats ; statistics ; mass spectrometry ; proteomics ; Controlled mixture ; MassIVE.quant reviewed - Platinum
Principal Investigators: (in alphabetical order) |
Eduard Sabid�, CRG/UPF Proteomics Unit, Centre de Regulaci� Gen�mica, 08003 Barcelona, Spain, N/A |
Submitting User: | ccms |
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Owner | Reanalyses | |
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