MassIVE MSV000092983

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Functional divergence of the paralogous RBM41 and U11/U12-65K proteins in the minor spliceosome

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Description

In this work, we identify RBM41 as a novel unique protein component of the minor spliceosome. RBM41 has no previously recognized cellular function but has been identified as a paralog of the U11/U12-65K protein, a known unique component of the minor spliceosome that functions during the early steps of minor intron recognition as a component of the U11/U12 di-snRNP. We show that both proteins use their highly similar C-terminal RRMs to bind to 3'-terminal stem-loops in U12 and U6atac snRNAs with comparable affinity. Our BioID data indicate that the unique N-terminal domain of RBM41 is necessary for its association with complexes containing DHX8, an RNA helicase, which in the major spliceosome drives the release of mature mRNA from the spliceosome. [doi:10.25345/C5K64B409] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Interactome ; RBM41

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Principal Investigators:
(in alphabetical order) 		Markku Varjosalo, University of Helsinki, Finland
Submitting User: ichowdhu
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