MassIVE MSV000090738

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Effects of FSCN1 inactivation on protein expression in human adrenocortical cancer cells

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Description

Adrenocortical carcinoma (ACC) is a rare endocrine malignancy with a high risk of relapse and metastatisation. The actin-bundling protein fascin (FSCN1) is overexpressed in aggressive ACC and represents a reliable prognostic indicator. FSCN1 has been shown to synergize with the Rho/Rac GEF VAV2 in enhancing the invasion properties of ACC cancer cells. Based on those results, we investigated the effects of FSCN1 inactivation by CRISPR/Cas9 or pharmacological blockade on the invasive properties of ACC cells, both in vitro and in an in vivo metastatic ACC zebrafish model. Moreover, to assess the impact of FSCN1 inactivation on global gene and protein expression in H295R cells, we performed RNA-seq and proteomic profiling of control and FSCN1 knock-out (KO) clones. [doi:10.25345/C5HX15W12] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: human adrenocortical cancer ; human adrenocortical cancer cells ; proteomic profiling ; FSCN1

Contact

Principal Investigators:
(in alphabetical order) 		Enzo Lalli, Institut de Pharmacologie Moleculaire et Cellulaire, CNRS, Universite Cote d Azur, INSERM, Valbonne, France
Submitting User: FPP_34

Publications

Ruggiero C, Tamburello M, Rossini E, Zini S, Durand N, Cantini G, Cioppi F, Hantel C, Kiseljak-Vassiliades K, Wierman ME, Landwehr LS, Weigand I, Kurlbaum M, Zizioli D, Turtoi A, Yang S, Berruti A, Luconi M, Sigala S, Lalli E.
FSCN1 as a new druggable target in adrenocortical carcinoma.
Int J Cancer. Epub 2023 Mar 27.

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