MassIVE MSV000099982

Partial Public PXD071044

Proteomics Analysis of Cerebrospinal Fluid in Survivors of Childhood Acute Lymphoblastic Leukemia

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Description

Mass spectrometry-based proteomic profiling of cerebrospinal fluid (CSF) in large patient cohorts, particularly among childhood cancer survivors, remains scarce, limiting opportunities for benchmarking, method development, and validation. Here, we present a longitudinal CSF proteomics dataset from survivors of childhood acute lymphoblastic leukemia (ALL). Samples were collected from 178 patients enrolled in the TOTXVI therapeutic protocol at two timepoints: diagnosis (pre-treatment) and during the consolidation phase of chemotherapy (totaling 356 samples). CSF proteomes were profiled using tandem-mass-tag (TMT) labeling coupled with extensive fractionation and high-resolution liquid chromatography-tandem mass spectrometry (LC/LC-MS/MS). The dataset includes quantitative profiles for more than 3,000 confidently identified proteins. This resource enables the investigation of chemotherapy-induced alterations in the CSF proteome and provides a valuable resource for studying the molecular mechanisms of neurotoxicity, identifying biomarkers of adverse late effects, and guiding the development of neuroprotective strategies in childhood cancer survivors. [doi:10.25345/C5Q52FS3N] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Cerebrospinal Fluid ; Childhood Acute Lymphoblastic Leukemia ; Chemotherapy ; childhood cancer survivors ; LC/LC-MS/MS ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Junmin Peng, St. Jude Children's Research Hospital, USA
Submitting User: CSF_ALL_2025
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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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